5OHM
K33-specific affimer bound to K33 diUb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2014-12-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.917410 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.866, 149.604, 73.788 |
Unit cell angles | 90.00, 110.44, 90.00 |
Refinement procedure
Resolution | 48.460 - 3.800 |
R-factor | 0.2352 |
Rwork | 0.233 |
R-free | 0.28250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ohv |
RMSD bond length | 0.003 |
RMSD bond angle | 0.655 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.460 | 4.250 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.237 | 0.779 |
Number of reflections | 10962 | |
<I/σ(I)> | 3.8 | 1.5 |
Completeness [%] | 97.9 | 99.2 |
Redundancy | 3.3 | 3.4 |
CC(1/2) | 0.665 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 295 | 20% PEG3350 0.2M potassium thiocyanate |