5OH8
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Rolipram
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.339, 59.523, 88.031 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.300 - 1.950 |
R-factor | 0.1803 |
Rwork | 0.178 |
R-free | 0.22024 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v2y |
RMSD bond length | 0.014 |
RMSD bond angle | 1.813 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.300 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.076 | 0.650 |
Number of reflections | 22598 | 3569 |
<I/σ(I)> | 10.8 | 1.91 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 4.64 | 4.64 |
CC(1/2) | 1.000 | 0.780 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M sodium acetate pH 4.6, 15 %(W/V) PEG 20000 |