5OH8

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Rolipram

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	57.339, 59.523, 88.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.300 - 1.950
R-factor	0.1803
Rwork	0.178
R-free	0.22024
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v2y
RMSD bond length	0.014
RMSD bond angle	1.813
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.300	2.070
High resolution limit [Å]	1.950	1.950
Rmerge	0.076	0.650
Number of reflections	22598	3569
<I/σ(I)>	10.8	1.91
Completeness [%]	99.8	99.4
Redundancy	4.64	4.64
CC(1/2)	1.000	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M sodium acetate pH 4.6, 15 %(W/V) PEG 20000