5OH6

Legionella pneumophila RidL N-terminal domain lacking beta hairpin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	40.100, 58.050, 94.420
Unit cell angles	90.00, 98.28, 90.00

Refinement procedure

Resolution	49.309 - 2.050
R-factor	0.2037
Rwork	0.202
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oh6
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.309	49.309	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.058	0.025	0.785
Rmeas	0.063	0.028	0.848
Number of reflections	26742	334	1989
<I/σ(I)>	15.68	59.4	2.32
Completeness [%]	98.5	99.7	98.6
Redundancy	6.868	6.722	6.857
CC(1/2)	0.999	0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% (w/v) PEG 1500