5OH3
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Ethosuximide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-09-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.880, 59.790, 88.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.330 - 2.100 |
R-factor | 0.18199 |
Rwork | 0.179 |
R-free | 0.23066 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v2y |
RMSD bond length | 0.009 |
RMSD bond angle | 1.327 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.400 | 2.220 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.065 | 0.486 |
Number of reflections | 18109 | 2816 |
<I/σ(I)> | 13.4 | 2.29 |
Completeness [%] | 99.2 | 97.1 |
Redundancy | 3.39 | 3.24 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M sodium acetate pH 4.6, 15 %(W/V) PEG 20000 |