5OH1
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Aminoglutethimide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.831, 60.446, 88.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.500 - 1.700 |
| R-factor | 0.16846 |
| Rwork | 0.166 |
| R-free | 0.20972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.534 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.590 |
| Number of reflections | 34222 | 5404 |
| <I/σ(I)> | 15.4 | 1.97 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 3.45 | 3.42 |
| CC(1/2) | 1.000 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M sodium acetate PH 4.6, 15 %(W/V) PEG 20000 |
