5OGH
Structure of RNase A at high resolution (1.16 A) in complex with 3'-CMP and sulphate ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.060, 64.060, 64.058 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.648 - 1.160 |
| R-factor | 0.1213 |
| Rwork | 0.121 |
| R-free | 0.13540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kf3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.490 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.650 | 1.220 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.046 | 0.470 |
| Number of reflections | 52994 | |
| <I/σ(I)> | 20.9 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.54 M ammonium sulphate, 1.25 M sodium chloride, 0.1 M sodium acetate pH 6.0 |
