5OFN

Crystal structure of the heterotrimeric PriSLX primase from S. solfataricus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	205.052, 36.348, 110.941
Unit cell angles	90.00, 96.91, 90.00

Refinement procedure

Resolution	46.170 - 3.005
R-factor	0.2439
Rwork	0.241
R-free	0.29790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zt2
RMSD bond length	0.001
RMSD bond angle	0.374
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.170	3.113
High resolution limit [Å]	3.005	3.005
Rmerge	0.117	0.809
Rpim	0.077	0.520
Number of reflections	16582	1556
<I/σ(I)>	9.18	1.86
Completeness [%]	98.6	95.17
Redundancy	3.2	3.3
CC(1/2)	0.990	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	12% PEG 3350, 200 mM CaCl2, 0.5% n-octyl-beta-D-glucoside