5OF9

Crystal structure of human MORC2 (residues 1-603)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.972636
Spacegroup name	P 1 21 1
Unit cell lengths	66.170, 127.930, 80.190
Unit cell angles	90.00, 101.22, 90.00

Refinement procedure

Resolution	64.906 - 1.807
R-factor	0.1624
Rwork	0.161
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ix2
RMSD bond length	0.012
RMSD bond angle	1.108
Data reduction software	XDS (1.1.7)
Data scaling software	Aimless (1.1.7)
Phasing software	PHASER
Refinement software	PHENIX ((1.12rc2_2821: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.658	78.658	1.838
High resolution limit [Å]	1.807	4.905	1.807
Rmerge	0.082	0.053	0.901
Rpim	0.049	0.032	0.555
Number of reflections	118149	5994	5948
<I/σ(I)>	13.6	28.8	2.1
Completeness [%]	98.9	98.5	98.3
Redundancy	6.7	6.3	6.3
CC(1/2)	0.998	0.996	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 M bicine/Trizma pH 8.5 9% PEG 4000 18% glycerol 0.02 M each of the following: 1,6-hexanediol; 1-butanol; RS-1,2 propanediol; 2-propanol; 1,4-butanediol; 1,3-propanediol Based on Morpheus crystal screen