5OF1
The structural versatility of TasA in B. subtilis biofilm formation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.728, 43.149, 51.744 |
| Unit cell angles | 89.92, 91.86, 90.01 |
Refinement procedure
| Resolution | 40.710 - 1.560 |
| R-factor | 0.15273 |
| Rwork | 0.152 |
| R-free | 0.17523 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.842 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.710 | 1.614 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.078 | 0.416 |
| Number of reflections | 47368 | 4658 |
| <I/σ(I)> | 13.33 | 2.73 |
| Completeness [%] | 96.3 | 93.31 |
| Redundancy | 4.1 | 4.2 |
| CC(1/2) | 0.996 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293.15 | 34% PEG2000 MME, 0.2M lithium salicylate, 0.1M ammonium sulfate, 0.1M sodium acetate pH 4.8 |
