5OF1
The structural versatility of TasA in B. subtilis biofilm formation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9797 |
Spacegroup name | P 1 |
Unit cell lengths | 40.728, 43.149, 51.744 |
Unit cell angles | 89.92, 91.86, 90.01 |
Refinement procedure
Resolution | 40.710 - 1.560 |
R-factor | 0.15273 |
Rwork | 0.152 |
R-free | 0.17523 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.842 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.710 | 1.614 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.078 | 0.416 |
Number of reflections | 47368 | 4658 |
<I/σ(I)> | 13.33 | 2.73 |
Completeness [%] | 96.3 | 93.31 |
Redundancy | 4.1 | 4.2 |
CC(1/2) | 0.996 | 0.921 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293.15 | 34% PEG2000 MME, 0.2M lithium salicylate, 0.1M ammonium sulfate, 0.1M sodium acetate pH 4.8 |