5OEZ
Crystal structure of Leishmania major fructose-1,6-bisphosphatase in apo form.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-13 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.260, 162.560, 170.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.770 - 2.410 |
R-factor | 0.18886 |
Rwork | 0.187 |
R-free | 0.22536 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fbp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.495 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.770 | 2.496 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.094 | |
Number of reflections | 59755 | |
<I/σ(I)> | 9.2 | 2.9 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 290.15 | 20% w/v PEG 3,350, 0.05 M citric acid, 0.05 M Bis-Tris propane |