5OEE
Structure of large terminase from the thermophilic bacteriophage D6E (Crystal form 3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 100.757, 102.628, 103.114 |
Unit cell angles | 90.72, 117.39, 119.37 |
Refinement procedure
Resolution | 45.690 - 2.600 |
R-factor | 0.205 |
Rwork | 0.202 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.119 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.690 | 45.690 | 2.650 |
High resolution limit [Å] | 2.600 | 14.250 | 2.600 |
Rmerge | 0.103 | 0.050 | 1.153 |
Rmeas | 0.122 | 0.059 | 1.367 |
Rpim | 0.065 | 0.031 | 0.726 |
Number of reflections | 91259 | ||
<I/σ(I)> | 7.7 | ||
Completeness [%] | 97.7 | 94.8 | 93.8 |
Redundancy | 3.5 | 3.5 | 3.4 |
CC(1/2) | 0.995 | 0.996 | 0.331 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.2 M Ammonium Sulfate, 0.1 M HEPES pH 8.0 |