5OEB
Structure of large terminase from the thermophilic bacteriophage D6E in complex with ADP (Crystal form 3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 100.878, 102.859, 103.029 |
| Unit cell angles | 91.13, 117.16, 119.17 |
Refinement procedure
| Resolution | 45.800 - 3.100 |
| R-factor | 0.2393 |
| Rwork | 0.237 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.800 | 45.800 | 3.190 |
| High resolution limit [Å] | 3.100 | 13.140 | 3.100 |
| Rmerge | 0.164 | 0.030 | 0.832 |
| Rmeas | 0.232 | 0.043 | 1.177 |
| Rpim | 0.164 | 0.030 | 0.832 |
| Total number of observations | 95733 | 1171 | 7439 |
| Number of reflections | 53693 | 646 | 4293 |
| <I/σ(I)> | 4 | 15 | 1 |
| Completeness [%] | 96.4 | 88.6 | 94 |
| Redundancy | 1.8 | 1.8 | 1.7 |
| CC(1/2) | 0.972 | 0.996 | 0.336 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.2 M Ammonium Sulfate, 0.1 M HEPES pH 8.0 |
