5OE9
Structure of large terminase from the thermophilic bacteriophage D6E in complex with sulfate (Crystal form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9282 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.297, 102.249, 110.282 |
Unit cell angles | 90.00, 124.87, 90.00 |
Refinement procedure
Resolution | 46.650 - 2.400 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.23310 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.294 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.650 | 46.650 | 2.440 |
High resolution limit [Å] | 2.380 | 10.910 | 2.380 |
Rmerge | 0.062 | 0.020 | 1.131 |
Rmeas | 0.071 | 0.023 | 1.313 |
Rpim | 0.034 | 0.011 | 0.652 |
Total number of observations | 268569 | ||
Number of reflections | 64217 | ||
<I/σ(I)> | 13.5 | ||
Completeness [%] | 97.1 | 96.6 | 67.4 |
Redundancy | 4.2 | 4.1 | 3.3 |
CC(1/2) | 0.999 | 0.999 | 0.420 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.2 M Ammonium Sulfate, 0.1 M HEPES pH 8.0 |