5OE8
Structure of large terminase from the thermophilic bacteriophage D6E (Crystal form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9173 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.085, 101.584, 108.500 |
| Unit cell angles | 90.00, 124.53, 90.00 |
Refinement procedure
| Resolution | 46.050 - 2.200 |
| R-factor | 0.1886 |
| Rwork | 0.186 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.050 | 46.050 | 2.240 |
| High resolution limit [Å] | 2.200 | 11.220 | 2.200 |
| Rmerge | 0.080 | 0.040 | 1.065 |
| Rmeas | 0.091 | 0.046 | 1.227 |
| Rpim | 0.044 | 0.024 | 0.601 |
| Total number of observations | 335994 | 2120 | 17051 |
| Number of reflections | 80788 | ||
| <I/σ(I)> | 11.6 | 36.5 | 1.4 |
| Completeness [%] | 99.5 | 93.9 | 94.6 |
| Redundancy | 4.2 | 3.5 | 3.9 |
| CC(1/2) | 0.998 | 0.996 | 0.479 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Ammonium Sulfate, 0.1 M HEPES pH 6.5-8.5. |
