5ODU
PllA lectin, monosaccharide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.904, 103.342, 76.086 |
| Unit cell angles | 90.00, 93.05, 90.00 |
Refinement procedure
| Resolution | 51.671 - 1.560 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l7l |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.575 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.670 | 1.616 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.099 | 1.261 |
| Rmeas | 0.112 | 1.427 |
| Rpim | 0.052 | 0.662 |
| Number of reflections | 133175 | 13311 |
| <I/σ(I)> | 11.19 | 1.22 |
| Completeness [%] | 96.2 | 95.63 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Ammonium acetate,0.1M BIS-Tris ph 5.5, 25 % w/v PEG 3350 |
