5ODS
Structure of a phosphoprotein-protein complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.520, 120.040, 123.130 |
| Unit cell angles | 90.00, 95.72, 90.00 |
Refinement procedure
| Resolution | 61.258 - 3.090 |
| R-factor | 0.2231 |
| Rwork | 0.221 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.204 |
| Data reduction software | xia2 |
| Data scaling software | xia2 (0.4.0.291-ga780859-dials-1.1) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.260 | 3.140 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.084 | |
| Rmeas | 0.101 | |
| Rpim | 0.055 | |
| Number of reflections | 60689 | 3053 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8 | 291 | PEG 6000, sodium chloride, Tris |
