5ODP

Salinibacter ruber Single-Strand Binding protein D17K D71K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 42 21 2
Unit cell lengths	102.587, 102.587, 61.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.911 - 2.535
R-factor	0.2186
Rwork	0.217
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5odn
RMSD bond length	0.003
RMSD bond angle	0.657
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.535	2.535
Rmeas	0.122	1.244
Number of reflections	11326	1764
<I/σ(I)>	17.59	2.19
Completeness [%]	99.6	98.9
Redundancy	12.7
CC(1/2)	0.999	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 10k 9.9%