5ODO

Crystal Structure of the Oleate hydratase of Rhodococcus erythropolis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98141
Spacegroup name	P 65 2 2
Unit cell lengths	261.747, 261.747, 127.304
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.466 - 2.640
R-factor	0.19
Rwork	0.189
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ia5
RMSD bond length	0.011
RMSD bond angle	0.842
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.640
Number of reflections	75230
<I/σ(I)>	13.66
Completeness [%]	99.8
Redundancy	22.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	100 mM Bis-Tris/HCl 300 mM Magnesium formate 5% (v/v) glycerol