5ODN

Salinibacter ruber Single-Strand Binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	C 2 2 21
Unit cell lengths	106.470, 123.500, 205.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.255 - 2.598
R-factor	0.1923
Rwork	0.190
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eyg
RMSD bond length	0.007
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.598	2.598
Rmeas	0.080	0.594
Number of reflections	79148	12799
<I/σ(I)>	12.71	2
Completeness [%]	98.3	98.4
Redundancy	2.3
CC(1/2)	0.997	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350 27% w/v, Tris-HCl pH 8.0, MgCl2 0.25M, ammonium sulphate 1M