5ODL

Single-stranded DNA-binding protein from bacteriophage Enc34 in complex with ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91840
Spacegroup name	P 61
Unit cell lengths	43.489, 43.489, 179.106
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.660 - 1.560
R-factor	0.14336
Rwork	0.141
R-free	0.19148
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5odk
RMSD bond length	0.011
RMSD bond angle	1.523
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.600	1.560
High resolution limit [Å]	1.560	1.560
Rmerge	0.071	0.679
Rpim	0.036	0.344
Number of reflections	27151	3942
<I/σ(I)>	9.5	2.1
Completeness [%]	99.7	99.7
Redundancy	4.8	4.7
CC(1/2)	0.999	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M HEPES, 30% Jeffamine ED-2001