5ODK

Single-stranded DNA-binding protein from bacteriophage Enc34, C-terminal truncation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97181
Spacegroup name	P 1 21 1
Unit cell lengths	50.770, 103.620, 59.290
Unit cell angles	90.00, 111.09, 90.00

Refinement procedure

Resolution	29.610 - 1.340
R-factor	0.15215
Rwork	0.150
R-free	0.18633
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5odj
RMSD bond length	0.011
RMSD bond angle	1.627
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.610	1.420
High resolution limit [Å]	1.340	1.340
Rmerge	0.047	0.369
Rpim	0.032	0.266
Number of reflections	126056	18326
<I/σ(I)>	12.6	2.8
Completeness [%]	99.3	99.1
Redundancy	3.1	2.7
CC(1/2)	0.997	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.04 M potassium dihydrogen phosphate, 16% PEG 8000, 20% glycerol