5ODJ

Single-stranded DNA-binding protein from bacteriophage Enc34

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97671
Spacegroup name	P 21 21 21
Unit cell lengths	38.080, 67.200, 76.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.780 - 1.500
R-factor	0.15819
Rwork	0.157
R-free	0.19255
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.571
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.780	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.104	0.770
Rpim	0.062	0.464
Number of reflections	32072	4638
<I/σ(I)>	6.9	2.5
Completeness [%]	99.4	100
Redundancy	3.6	3.6
CC(1/2)	0.989	0.579

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Bis-tris methane, 0.3 M MgCl2, 20% PEG 3350