5ODG
Crystal structure of Smad3-MH1 bound to the GGCT site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9700 |
| Spacegroup name | I 41 |
| Unit cell lengths | 104.990, 104.990, 72.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 2.120 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.24200 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.130 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmeas | 0.049 | 0.663 |
| Number of reflections | 22058 | 292 |
| <I/σ(I)> | 14.84 | 1.7 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 3.77 | 3.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.02 M sodium potassium phosphate, 0.1 M BisTris propane pH 6.5, 20% PEG 3350 |
