5OD9
Structure of the engineered metalloesterase MID1sc9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-22 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000030 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.820, 104.959, 33.604 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.000 - 1.130 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.480 | 1.200 |
High resolution limit [Å] | 1.130 | 1.130 |
Rmerge | 0.096 | 1.500 |
Number of reflections | 58851 | |
<I/σ(I)> | 13.58 | 0.82 |
Completeness [%] | 99.1 | 94.8 |
Redundancy | 11.8 | |
CC(1/2) | 0.999 | 0.326 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.92 | 293 | 100 mM BIS-TRIS-propane pH 7.92, 30% (w/v) polyethylene glycol 4000, 431 mM MgCl2. |