5OD4
Avr2 effector protein from the fungal plant pathogen Fusarium oxysporum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9168 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.410, 51.730, 74.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.100 |
R-factor | 0.14026 |
Rwork | 0.139 |
R-free | 0.16899 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.823 |
Data reduction software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.540 |
High resolution limit [Å] | 1.100 |
Number of reflections | 53312 |
<I/σ(I)> | 26.1 |
Completeness [%] | 99.8 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES, pH 6.5; 25% (w/v) PEG 8K |