5OD2
Crystal structure of ADP-dependent glucokinase from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 31 |
| Unit cell lengths | 154.565, 154.565, 50.483 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.000 - 1.980 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3drw |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.240 | 2.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.180 | 0.890 |
| Number of reflections | 93701 | |
| <I/σ(I)> | 8.71 | |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 5.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium chloride 0.1 M BIS-TRIS, pH 5.5 25 % PEG 3350 |
