5OCC

Crystal structure of CD32b (Fc Gamma Receptor IIb) in complex with Human IgG1 Fab fragment (6G08)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	70.230, 75.020, 134.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	134.950 - 2.500
R-factor	0.19585
Rwork	0.192
R-free	0.26077
RMSD bond length	0.021
RMSD bond angle	2.178
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	134.950
High resolution limit [Å]	2.500
Rmerge	0.134
Number of reflections	23884
<I/σ(I)>	9.6
Completeness [%]	99.5
Redundancy	5.5
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1M MES, 0.01M Zinc Chloride, 20% PEG 6000, pH 6.0