5OC1
Crystal structure of aryl-alcohol oxidase from Pleurotus eryngii in complex with p-anisic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 179.326, 179.326, 160.177 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.300 - 2.300 |
| R-factor | 0.17583 |
| Rwork | 0.174 |
| R-free | 0.19496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fim |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.661 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.990 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.133 | |
| Number of reflections | 67615 | 9716 |
| <I/σ(I)> | 28.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 40.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1 M sodium acetate, pH 4.5 and 1.0 M di-ammonium hydrogen phosphate |
