5OBZ
low resolution structure of the p34ct/p44ct minimal complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 138.212, 138.212, 95.235 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.200 - 3.700 |
| R-factor | 0.19843 |
| Rwork | 0.196 |
| R-free | 0.24623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nus |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.610 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.200 |
| High resolution limit [Å] | 3.700 |
| Rmerge | 0.200 |
| Number of reflections | 6120 |
| <I/σ(I)> | 9.5 |
| Completeness [%] | 99.9 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 15% PEG 20,000 100 mM MES pH 6.5 |
