5OBZ

low resolution structure of the p34ct/p44ct minimal complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 63 2 2
Unit cell lengths	138.212, 138.212, 95.235
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.200 - 3.700
R-factor	0.19843
Rwork	0.196
R-free	0.24623
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nus
RMSD bond length	0.011
RMSD bond angle	1.610
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.200
High resolution limit [Å]	3.700
Rmerge	0.200
Number of reflections	6120
<I/σ(I)>	9.5
Completeness [%]	99.9
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	15% PEG 20,000 100 mM MES pH 6.5