5OBF
fAb. AbVance: increasing our knowledge of antibody structural space to enable faster and better decision-making in antibody drug discovery.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 |
| Unit cell lengths | 132.397, 132.397, 56.914 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.490 - 1.920 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.21000 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.960 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.063 | 0.529 |
| Number of reflections | 37566 | 4686 |
| <I/σ(I)> | 20.1 | 3.6 |
| Completeness [%] | 97.6 | 83.8 |
| Redundancy | 7.2 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M Tris pH8.5 2M Ammonium Sulfate 15% glycerol used as cryoprotectant. |
