5OB5
fAb complex with GroBeta. AbVance: increasing our knowledge of antibody structural space to enable faster and better decision-making in antibody drug discovery.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.190, 60.210, 68.250 |
| Unit cell angles | 90.00, 110.44, 90.00 |
Refinement procedure
| Resolution | 24.350 - 1.650 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.069 | 0.549 |
| Number of reflections | 70965 | 7047 |
| <I/σ(I)> | 18.7 | 2.6 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 1.6M ammonium sulfate, 0.5M lithium chloride. 5-30% glycerol added as cryoprotectant |
