5OB3
iSpinach aptamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.80002 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.700, 48.500, 30.100 |
| Unit cell angles | 90.00, 91.86, 90.00 |
Refinement procedure
| Resolution | 46.166 - 2.004 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q9r.pdb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.170 | 2.120 |
| High resolution limit [Å] | 2.004 | 2.004 |
| Rmerge | 0.103 | |
| Rmeas | 0.106 | |
| Number of reflections | 27458 | 2470 |
| <I/σ(I)> | 17.6 | 1.9 |
| Completeness [%] | 99.0 | 91.7 |
| Redundancy | 15.6 | 9.2 |
| CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 40 mM NaCl, 40 mM Sodium cacodylate pH 7.0, 32% MPD, 12 mM Spermine |
