5O9K

Crystal structure of Murine Histmaine-Releasing Factor (HRF/TCTP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949 A
Spacegroup name	P 21 21 2
Unit cell lengths	69.530, 99.850, 57.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.059 - 4.014
R-factor	0.3017
Rwork	0.300
R-free	0.34160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yz1
RMSD bond length	0.001
RMSD bond angle	0.361
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.060	4.630
High resolution limit [Å]	4.010	4.010
Rmerge	0.271	1.892
Rpim	0.081	0.554
Number of reflections	3606	1200
<I/σ(I)>	9	2.1
Completeness [%]	98.5	95.8
Redundancy	11.6	12
CC(1/2)	0.997	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	291	0.1M Tris-HCl pH8.4, 23% (w/v) PEG 2000 MME and 0.01M nickel chloride