5O9E
Crystal structure of the Imp4-Mpp10 complex from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-06 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8729 |
| Spacegroup name | P 64 |
| Unit cell lengths | 112.890, 112.890, 44.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.883 - 1.884 |
| R-factor | 0.1853 |
| Rwork | 0.184 |
| R-free | 0.21920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5by8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.935 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.883 |
| High resolution limit [Å] | 1.880 |
| Number of reflections | 26155 |
| <I/σ(I)> | 9.15 |
| Completeness [%] | 100.0 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20-30 % Ethylene glycol |
