5O8P
The crystal structure of DfoA bound to FAD, the desferrioxamine biosynthetic pathway cadaverine monooxygenase from the fire blight disease pathogen Erwinia amylovora
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 164.090, 164.090, 168.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 117.570 - 2.750 |
R-factor | 0.2013 |
Rwork | 0.200 |
R-free | 0.22398 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tlx |
RMSD bond length | 0.009 |
RMSD bond angle | 1.434 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 117.570 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmeas | 0.066 | 0.670 |
Number of reflections | 60324 | 4397 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 100.0 | |
Redundancy | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1 M HEPES, 1.45 M ammonium sulphate |