5O8I
Crystal structure of human histidine triad nucleotide-binding protein 1 (hHINT1) crystallized at P212121 space group, and refined to 1.27 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.031, 76.364, 80.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.430 - 1.270 |
R-factor | 0.10183 |
Rwork | 0.100 |
R-free | 0.13326 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tw2 |
RMSD bond length | 0.030 |
RMSD bond angle | 2.652 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.31) |
Phasing software | MOLREP (11.4.06) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.430 | 1.290 |
High resolution limit [Å] | 1.270 | 1.270 |
Rmerge | 0.044 | 0.535 |
Rpim | 0.038 | 0.461 |
Number of reflections | 74711 | 3586 |
<I/σ(I)> | 14.1 | |
Completeness [%] | 98.6 | 95.8 |
Redundancy | 4.1 | 3.8 |
CC(1/2) | 0.999 | 0.775 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281 | 20% w/v PEG 3350, 0.1 M Bis-Tris Propane pH 8.5, 0.2 M Sodium/Potassium Phosphate |