5O8C
Composite structure of rsEGFP2 1ps following 400nm-laser irradiation of the off-state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2015-05-01 |
| Detector | CS-PAD CXI-2 |
| Wavelength(s) | 1.3 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.530, 62.850, 71.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.854 - 1.700 |
| R-factor | 0.1484 |
| Rwork | 0.147 |
| R-free | 0.17610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o8a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.045 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.854 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 25742 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 98.0 | |
| Redundancy | 516 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8 | 293 | 2 M ammonium sulphate, 20 mM NaCl, 120 mM HEPES |
