5O86

Mutant of claas II CPD photolyase from Methanosarcina mazei - W388F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979003
Spacegroup name	P 43 21 2
Unit cell lengths	69.720, 69.720, 243.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.299 - 1.687
R-factor	0.2056
Rwork	0.205
R-free	0.24300
RMSD bond length	0.010
RMSD bond angle	1.090
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	81.280
High resolution limit [Å]	1.687
Rmerge	0.200
Number of reflections	68915
<I/σ(I)>	5.3
Completeness [%]	99.9
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.5 M Lithium Sulfate, 7.5% (W/V) PEG 6000