5O7M

Single-shot pink beam serial crystallography: Phycocyanin (One chip, chip_1)

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	293
Detector technology	CCD
Collection date	2016-07-09
Detector	RAYONIX MX340-HS
Wavelength(s)	1.15-1.25
Spacegroup name	H 3 2
Unit cell lengths	187.800, 187.800, 60.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.917 - 2.460
R-factor	0.1475
Rwork	0.142
R-free	0.19620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jbo
RMSD bond length	0.004
RMSD bond angle	0.830
Data reduction software	Precognition
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.570
High resolution limit [Å]	2.460	2.460
Rmerge	0.073
Number of reflections	8931	462
<I/σ(I)>	47.57
Completeness [%]	60.0	25.16
Redundancy	11.7	11.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.4	293	PC was crystallized in 1.5 M Ammonium Sulfate solution, 25 mM MES pH 6.4. The protein crystals appear overnight in 1 mm inner diameter capillaries. The PC crystals size (30 to 40 um) is correlated to the protein concentration, which varied between 10-15 mg/ml.