5O7H
Structure of the Cascade-I-Fv complex from Shewanella putrefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 157.002, 65.894, 160.682 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 49.288 - 3.000 |
| R-factor | 0.2235 |
| Rwork | 0.221 |
| R-free | 0.26710 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.454 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.970 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.055 | |
| Number of reflections | 32876 | 3271 |
| <I/σ(I)> | 13.18 | 2.05 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 9.8 |
| CC(1/2) | 0.990 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 16% w/v PEG6000, 0.1 M Tris pH 8.0 and 20 mM 5-amino-2,4,6-triiodoisophthalic acid |
