5O77
Klebsiella pneumoniae OmpK35
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92 |
Spacegroup name | P 63 |
Unit cell lengths | 77.312, 77.312, 114.044 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.410 - 1.500 |
R-factor | 0.15763 |
Rwork | 0.157 |
R-free | 0.18476 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gcp |
RMSD bond length | 0.021 |
RMSD bond angle | 2.087 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.410 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rpim | 0.044 | 5.210 |
Number of reflections | 61689 | |
<I/σ(I)> | 14.3 | 2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.1 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 13% PEG 2000MME, 0.1 M calcium chloride, 0.1 M Tris |