5O6R
Structure of beta-phosphoglucomutase D10N mutant in complex with glucose-1-phosphate and aluminium tetrafluoride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-27 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.300, 54.900, 107.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.360 |
R-factor | 0.11652 |
Rwork | 0.115 |
R-free | 0.14898 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o08 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.741 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.780 | 1.440 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmerge | 0.027 | 0.110 |
Rpim | 0.077 | |
Number of reflections | 43148 | 16940 |
<I/σ(I)> | 24.5 | 7.4 |
Completeness [%] | 93.6 | 91.3 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 19-21 % PEG 3350 and 50 mM Mg acetate |