5O6P
Structure of beta-phosphoglucomutase D10N mutant in complex with glucose-1,6-bisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.750, 68.300, 83.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.20074 |
Rwork | 0.198 |
R-free | 0.25683 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wf9 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.224 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.080 | 0.260 |
Rpim | 0.170 | |
Number of reflections | 31189 | 4563 |
<I/σ(I)> | 10 | 4 |
Completeness [%] | 98.2 | 99.5 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 28-32 % PEG 4000 and 100 mM Na acetate |