5O6P
Structure of beta-phosphoglucomutase D10N mutant in complex with glucose-1,6-bisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.750, 68.300, 83.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.20074 |
| Rwork | 0.198 |
| R-free | 0.25683 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wf9 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.224 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.260 |
| Rpim | 0.170 | |
| Number of reflections | 31189 | 4563 |
| <I/σ(I)> | 10 | 4 |
| Completeness [%] | 98.2 | 99.5 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 28-32 % PEG 4000 and 100 mM Na acetate |
