5O6D

Structure of ScPif1 in complex with polydT and ATPgS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977760
Spacegroup name	P 21 21 21
Unit cell lengths	74.406, 90.783, 186.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.000 - 3.283
R-factor	0.2553
Rwork	0.252
R-free	0.32190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o6b
RMSD bond length	0.003
RMSD bond angle	0.697
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2733: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.000	3.400
High resolution limit [Å]	3.280	3.280
Rmerge	0.160	0.710
Number of reflections	17964	1952
<I/σ(I)>	7.62	2.27
Completeness [%]	89.7	99.54
Redundancy	6.5	6.2
CC(1/2)	0.990	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	298	100mM NaAc-HAc pH5.5 240mM NaF 14%-16% PEG8000