5O68
Crystal Structure of the Pseudomonas functional amyloid secretion protein FapF - R157A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.587, 125.708, 142.667 |
| Unit cell angles | 87.09, 84.70, 89.92 |
Refinement procedure
| Resolution | 91.730 - 3.080 |
| R-factor | 0.32175 |
| Rwork | 0.319 |
| R-free | 0.36950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5065 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.543 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.700 | 3.130 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rpim | 0.103 | 0.500 |
| Number of reflections | 94515 | |
| <I/σ(I)> | 3.49 | 1.2 |
| Completeness [%] | 99.0 | 97.1 |
| Redundancy | 3.35 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0/05 - 0.1 M sodium citrate, 0.1 M sodium chloride, 20-30 % PEG400 |
