5O65
Crystal Structure of the Pseudomonas functional amyloid secretion protein FapF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9798 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.260, 124.731, 80.359 |
Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
Resolution | 2.640 - 2.500 |
R-factor | 0.22343 |
Rwork | 0.222 |
R-free | 0.25928 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.736 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.380 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.059 | 0.400 |
Number of reflections | 48100 | |
<I/σ(I)> | 10.8 | 2.1 |
Completeness [%] | 99.5 | 97.8 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 - 0.1 M sodium citrate, 0.1 M sodium chloride, 20-30 % PEG 400 |