5O60
Structure of the 50S large ribosomal subunit from Mycobacterium smegmatis
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.18 |
| Software | EMAN Boxer,RELION,PHENIX,CTFFIND |
| Symmetry | C1 |
| CTF correction | NONE |
