5O5C
The crystal structure of DfoJ, the desferrioxamine biosynthetic pathway lysine decarboxylase from the fire blight disease pathogen Erwinia amylovora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 32 |
| Unit cell lengths | 269.791, 269.791, 56.194 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 233.650 - 2.100 |
| R-factor | 0.16604 |
| Rwork | 0.165 |
| R-free | 0.18662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qma |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.822 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.960 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.084 | 0.633 |
| Number of reflections | 134700 | |
| <I/σ(I)> | 19.7 | 3.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 55% V/V polipropylene glycol 400 |
