5O55
Crystal structure of the human BRPF1 bromodomain in complex with BZ047
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999989 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.695, 60.695, 63.147 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.353 - 1.450 |
R-factor | 0.1793 |
Rwork | 0.177 |
R-free | 0.20110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lc2 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.755 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.570 | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.031 | 0.468 |
Number of reflections | 24259 | 3462 |
<I/σ(I)> | 31.2 | |
Completeness [%] | 99.8 | 99 |
Redundancy | 9.4 | 8.1 |
CC(1/2) | 1.000 | 0.943 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Bis-tris propane, pH6.5, 0.15 M Sodium Nitrate, 20% PEG3350 |