5O51
AfRom2 CNH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9282 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.980, 93.710, 52.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.870 - 2.010 |
R-factor | 0.20692 |
Rwork | 0.205 |
R-free | 0.24824 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.917 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.870 |
High resolution limit [Å] | 2.010 |
Rmerge | 0.095 |
Number of reflections | 26362 |
<I/σ(I)> | 13.3 |
Completeness [%] | 99.8 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.02 M L-Na-Glutamate; 0.02 M Alanine (racemic);0.02M Glycine; 0.02M Lysine HCl (racemic); 0.02M Serine (racemic);0.1 M tris Bicine buffer (pH 8.5); 20% v/v Ethylene glycol; 10 % w/v PEG 8000 |